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Anti-KLF4 Antibody (Ser457) LS-C98591

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KLF4 Antibodies

Anti-KLF4 Antibody (aa1-170, clone AT4E6) IHC-plus™ LS-B2966
Mouse Monoclonal [clone AT4E6] (IgG1,k) to Human KLF4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa401-450) IHC-plus™ LS-B5641
Rabbit Polyclonal (IgG) to Human KLF4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa2-470) LS-C149986
Goat Polyclonal (IgG) to Human KLF4
Human
IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human KLF4
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human KLF4
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

KLF4 antibody was raised against kLH-conjugated synthetic peptide between amino acids 481-504 from human KLF4.
LS-E7044 - Lyophilized - 100 µg - $145.00
Ser457

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About KLF4

O43474 NM_004235 NP_004226.3

KLF4 Antibody, EZF Antibody, GKLF Antibody, Kruppel-like factor 4 (gut) Antibody, Hezf Antibody, Krueppel-like factor 4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cells stained KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using KLF4 Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Western blot

Western blot
Western blot of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 12/4/2016

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