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Anti-KLF4 Antibody LS-C150213


Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular KLF4 Antibodies

Anti-KLF4 Antibody (aa1-170, clone AT4E6) IHC-plus™ LS-B2966
Mouse Monoclonal [clone AT4E6] (IgG1,k) to Human KLF4
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-KLF4 Antibody (aa401-450) IHC-plus™ LS-B5641
Rabbit Polyclonal (IgG) to Human KLF4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-KLF4 Antibody (aa2-470) LS-C149986
Goat Polyclonal (IgG) to Human KLF4
IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse KLF4
ICC, Western blot, Chromatin Immunoprecipitation


Mouse KLF4
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • ICC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Chromatin Immunoprecipitation

Specificity and Use

KLF4 antibody was raised against e. coli-derived recombinant mouse KLF4 Ala1 1-Phe483 Accession # Q60793.
Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 60% cross-reactivity with recombinant human.
Western Blot, 0.1 µg/ml, Recombinant Mouse KLF4. Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 5-15 µg/ml.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
500 µl Reconstitute with sterile PBS.
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.

About KLF4

O43474 NM_004235 NP_004226.3

KLF4 Antibody, EZF Antibody, GKLF Antibody, Kruppel-like factor 4 (gut) Antibody, Hezf Antibody, Krueppel-like factor 4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.


KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti -Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.

Requested From: United States
Date Requested: 10/26/2016

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