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Anti-KLF4 Antibody (aa1-170, clone AT4E6) IHC-plus™ LS-B2966

Note: This antibody replaces LS-C93803

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

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93 KLF4 Antibodies

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Mouse Monoclonal [clone AT4E6] (IgG1,k) to Human KLF4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human KLF4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AT4E6]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

KLF4 antibody was raised against recombinant human KLF4 (1-170aa) purified from E. coli. NCBI Accession No.: NP_004226.3.
aa1-170
Immunohistochemistry: LS-B2966 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2966 was determined to be 20 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About KLF4

O43474 NM_004235 NP_004226.3

KLF4 Antibody, EZF Antibody, GKLF Antibody, Kruppel-like factor 4 (gut) Antibody, Hezf Antibody, Krueppel-like factor 4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.

Immunohistochemistry

Immunohistochemistry
Anti-KLF4 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2966 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2966 concentration 20 ug/ml.

Western blot

Western blot
Cell lysates of HeLa and A549 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KLF4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: 
Date Requested: 4/25/2017

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