Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 5F2] (IgG2b) to Human KIR2DS4
Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [5F2]
Protein G purified
Western blot (1:500 - 1:2000)
IHC - Paraffin
Specificity and Use
KIR2DS4 antibody was raised against recombinant human KIR2DS4 purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
KIR2DS4 Antibody, CD158I Antibody, KIR antigen 2DS4 Antibody, MHC class I NK cell receptor Antibody, NKAT8 Antibody, KKA3 Antibody, NKAT-8 Antibody, CD158i antigen Antibody, Cl-39 Antibody, KIR1D Antibody, KIR412 Antibody, p58 NK receptor CL-39/CL-17 Antibody
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2).
Recombinant human KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4 (each 100 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DS4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates recombinant human KIR2DS4 protein.
Requested From: United States
Date Requested: 2/27/2017