Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 2H6] (IgG2b) to Human KIR2DL4
Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [2H6]
Protein A purified
Western blot (1:500 - 1:2000)
IHC - Paraffin
Specificity and Use
KIR2DL4 antibody was raised against recombinant human KIR2DL4 purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2).
Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system. Lane 1: Extracellular domain of KIR2DL1(23-223aa); Lane 2: Extracellular domain of KIR2DL3(23-223aa); Lane 3: Extracellular domain of KIR2DL4(44-202aa).
Requested From: United States
Date Requested: 2/24/2017