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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KIR2DL1 / CD158a Antibody (clone NKVFS1, Azide‑free) LS‑C16153
CD158a antibody LS-C16153 is an unconjugated mouse monoclonal antibody to human CD158a (KIR2DL1). Validated for ELISA, Flow, Func, IP and WB.
Antibody
Catalog
Size
Price
LS-C16153-1000
1000 µg
$1,095

Popular KIR2DL1 / CD158a Products

Western blot of recombinant KIR2DL1 / CD158a.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Western blot of extracts of HepG2 cell lines, using KIR2DL1 antibody.
Species: Human
Applications: IHC, Western blot
Recombinant human protein KIR2DL1, KIR2DL3, KIR2DS4 and KIR2DL4 (each 50 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: KIR2DL1; Lane 2: KIR2DL3; Lane 3: KIR2DS4; Lane 4: KIR2DL4.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Functional Assay
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Product Description

CD158a antibody LS-C16153 is an unconjugated mouse monoclonal antibody to human CD158a (KIR2DL1). Validated for ELISA, Flow, Func, IP and WB.
About KIR2DL1 / CD158a
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). P43626 NM_014218 NP_055033.2

KIR2DL1 Antibody, 47.11 Antibody, Cl-42 Antibody, MHC class I NK cell receptor Antibody, NKAT-1 Antibody, p58 NK receptor CL-42/47.11 Antibody, NKAT Antibody, NKAT1 Antibody, p58.1 Antibody, CD158A Antibody, CD158a antigen Antibody, Killer Ig receptor Antibody, KIR-K64 Antibody, KIR221 Antibody


Specifications

Target
Human KIR2DL1 / CD158a
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [NKVFS1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:50 - 1:100)
  • ELISA
  • Functional Assay
Immunogen
Human natural killer (NK) cell lysate.
Specificity
Recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family: CD158a, CD158b and P50.3. Specifically recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). Reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
Usage
Suitable for use in Flow Cytometry, ELISA. Immunoprecipitation, Western Blot and Functional assays. Flow Cytometry: 1:50-1:100. Use 10 ul of the optimal working dilution to label 1E6 cells in 100ul. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Clone NKVFS1 is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
Presentation
PBS, pH 7.4, No preservatives added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/22/2018

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