CD158a antibody LS-C16153 is an unconjugated mouse monoclonal antibody to human CD158a (KIR2DL1). Validated for ELISA, Flow, Func, IP and WB.
Human KIR2DL1 / CD158a
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot
- Flow Cytometry (1:50 - 1:100)
- Functional Assay
Human natural killer (NK) cell lysate.
Recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family: CD158a, CD158b and P50.3. Specifically recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). Reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
Suitable for use in Flow Cytometry, ELISA. Immunoprecipitation, Western Blot and Functional assays. Flow Cytometry: 1:50-1:100. Use 10 ul of the optimal working dilution to label 1E6 cells in 100ul. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Clone NKVFS1 is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
PBS, pH 7.4, No preservatives added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.