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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Kinesin Heavy Chain / KIF5B Antibody (clone KN‑02) LS‑C46055
KIF5B antibody LS-C46055 is an unconjugated mouse monoclonal antibody to KIF5B (Kinesin Heavy Chain) from human, mouse, rat and other species. Validated for ICC.
Catalog
Size
Price
LS-C46055-100
100 µg
$290

Popular Kinesin Heavy Chain / KIF5B Products

Antibody
Species: Bovine, Chicken, Mammal, Sea urchin, Squid
Applications: IHC, ICC, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Anti-KIF5B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4501 concentration 10 ug/ml.
Species: Pig, Human, Mouse, Rat, Porcine
Applications: IHC, IHC - Paraffin, ICC
Anti-KIF5B antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2489 concentration 5 ug/ml.
Species: Human, Monkey, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Species: Human
Applications: IHC, Western blot, ELISA, Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Dog
Applications: IHC, IHC - Paraffin, Western blot

Product Description

KIF5B antibody LS-C46055 is an unconjugated mouse monoclonal antibody to KIF5B (Kinesin Heavy Chain) from human, mouse, rat and other species. Validated for ICC.
About Kinesin Heavy Chain / KIF5B
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. P33176 NM_004521 NP_004512.1

KIF5B Antibody, Kinesin 1 (110-120kD) Antibody, KINH Antibody, KNS Antibody, Kinesin family member 5B Antibody, Kinesin heavy chain Antibody, KNS1 Antibody, UKHC Antibody, Kinesin-1 heavy chain Antibody, Ubiquitous kinesin heavy chain Antibody


Specifications

Target
Pig Kinesin Heavy Chain / KIF5B
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Porcine (tested or 100% immunogen sequence identity)
Clonality
IgM Monoclonal [KN-02]
Conjugations
Unconjugated
Purification
Purified by precipitation and chromatography. Greater than 95% by SDS-PAGE
Modifications
Unmodified
Applications
  • ICC
Failed Applications
  • IHC - Paraffin
Immunogen
Enriched fraction of porcine brain kinesin.
Specificity
The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations.Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments.The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Presentation
TBS, pH 8.0, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.

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Images

Immunocytochemistry

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).
Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

Immunocytochemistry

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).
Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

Immunocytochemistry

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).
Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

Immunocytochemistry

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).
Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

Requested From: United States
Date Requested: 9/18/2018

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