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Anti-KIF23 / MKLP1 Antibody (aa32-81) IHC-plus™ LS-B1543

Note: This antibody replaces LS-C30088

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KIF23 / MKLP1 Antibodies

Anti-KIF23 / MKLP1 Antibody (aa32-81) IHC-plus™ LS-B1543
Rabbit Polyclonal (IgG) to Human KIF23 / MKLP1
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KIF23 / MKLP1 Antibody LS-C72730
Rabbit Polyclonal to Human KIF23 / MKLP1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-KIF23 / MKLP1 Antibody (Agarose) LS-C72731
Rabbit Polyclonal to Human KIF23 / MKLP1
Human
Immunoprecipitation
Agarose Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human KIF23 / MKLP1
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human KIF23 / MKLP1
Rabbit
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

KIF23 / MKLP1 antibody was raised against synthetic peptide located between aa32-81 of human KIF23 (Q02241, NP_004847). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Rabbit, Horse, Pig (100%); Bovine, Opossum (92%); Platypus (85%).
LS-E12013 - Lyophilized - 100 µg - $145.00
aa32-81
Human KIF23 / MKLP1
Immunohistochemistry: LS-B1543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1543 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About KIF23 / MKLP1

Q02241 NM_004856 NP_004847.2

KIF23 Antibody, Kinesin family member 23 Antibody, Kinesin-like protein KIF23 Antibody, Mitotic kinesin-like 1 Antibody, MKLP1 Antibody, MKLP-1 Antibody, Kinesin-like protein 5 Antibody, KNSL5 Antibody, CHO1 Antibody, Mitotic kinesin-like protein 1 Antibody

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.

Immunohistochemistry

Immunohistochemistry
Anti-KIF23 / MKLP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1543 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
KIF23 / MKLP1 antibody LS-B1543 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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