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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KIF21A Antibody (aa551‑600) IHC‑plus™ LS‑B1542 Best Seller
Note: This antibody replaces LS-C30432
KIF21A antibody LS-B1542 is an unconjugated rabbit polyclonal antibody to KIF21A from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1542-50
50 µg
$485

Popular KIF21A Products

Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Western blot
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Western blot
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse, Human, Monkey, Dog, Hamster, Horse
Applications: Western blot

Product Description

KIF21A antibody LS-B1542 is an unconjugated rabbit polyclonal antibody to KIF21A from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About KIF21A
KIF21A is a member of the KIF4 subfamily of kinesin-like motor proteins. The encoded protein is characterized by an N-terminal motor domain a coiled-coil stalk domain and a C-terminal WD-40 repeat domain. This protein may be involved in microtubule dependent transport. Mutations in this gene are the cause of congenital fibrosis of extraocular muscles-1. Alternate splicing results in multiple transcript variants. Q7Z4S6 AF155117 AAD42883.1

KIF21A Antibody, CFEOM3B Antibody, FEOM3A Antibody, FEOM1 Antibody, KIAA1708 Antibody, Kinesin family member 21A Antibody, Kinesin-like protein KIF2 Antibody, Kinesin-like protein KIF21A Antibody, KIF2 Antibody, CFEOM1 Antibody


Specifications

Target
Human KIF21A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KIF21A antibody was raised against synthetic peptide located between aa551-600 of human KIF21A (Q7Z4S6, NP_060111). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine (100%); Elephant, Bat, Rabbit, Pig (92%); Mouse, Horse, Opossum (85%); Rat (78%).
Blocking Peptide
LS-E12006 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1542. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa551-600
Specificity
Human KIF21A
Usage
Immunohistochemistry: LS-B1542 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1542 was determined to be 5 ug/ml.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal Brain Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal Brain Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal Brain Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal Brain Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal Brain Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal Brain Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KIF21A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1542 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KIF21A antibody LS-B1542 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal Brain Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal Brain Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/19/2018

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