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Anti-KIAA0652 / ATG13 Antibody (phospho-Ser318) LS-C153855


Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

Most Popular KIAA0652 / ATG13 Antibodies

Anti-KIAA0652 / ATG13 Antibody (C-Terminus) IHC-plus™ LS-B5277
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Human, Mouse, Rat
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Anti-KIAA0652 / ATG13 Antibody (phospho-Ser318) LS-C153855
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Anti-KIAA0652 / ATG13 Antibody (C-Terminus) IHC-plus™ LS-B10936
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Anti-KIAA0652 / ATG13 Antibody LS-C331070
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Anti-KIAA0652 / ATG13 Antibody (aa1-30) IHC-plus™ LS-B11831
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100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human KIAA0652 / ATG13
Western blot, ELISA


Human KIAA0652 / ATG13
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • Western blot (1:1000)

Specificity and Use

KIAA0652 / ATG13 antibody was raised against this affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.
Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kD in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy. Joo JH, Dorsey FC, Joshi A, Hennessy-Walters KM, Rose KL, McCastlain K, Zhang J, Iyengar R, Jung CH, Suen DF, Steeves MA, Yang CY, Prater SM, Kim DH, Thompson CB, Youle RJ, Ney PA, Cleveland JL, Kundu M. Molecular cell. 2011 43:572-85. [PubMed:21855797] [PMC:PMC3485687]

About KIAA0652 / ATG13

O75143 NM_014741 NP_001136145.1

ATG13 Antibody, Autophagy-related protein 13 Antibody, KIAA0652 Antibody, Autophagy related 13 Antibody

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Western blot

Western blot
Western blot of affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.

Requested From: 
Date Requested: 4/25/2017

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