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Anti-KIAA0427 / CTIF Antibody (aa36-85) LS-C101872

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KIAA0427 / CTIF Antibodies

Anti-KIAA0427 / CTIF Antibody (aa36-85) LS-C101872
Rabbit Polyclonal (IgG) to Human KIAA0427 / CTIF
Human, Rat
Western blot
Unconjugated
Western blot Image
Anti-KIAA0427 / CTIF Antibody (aa71-120) LS-C110567
Rabbit Polyclonal to Human KIAA0427 / CTIF
Human, Monkey, Rat, Bovine, Pig, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-KIAA0427 / CTIF Antibody (aa71-120, HRP) LS-C441085
Rabbit Polyclonal to Human KIAA0427 / CTIF
Human, Chimpanzee, Gibbon, Monkey, Rat, Bovine, Pig, Rabbit
Western blot
HRP Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human KIAA0427 / CTIF
Human, Rat
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human KIAA0427 / CTIF
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
Monkey, Mouse, Bat, Bovine, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

KIAA0427 / CTIF antibody was raised against synthetic peptide located between aa36-85 of human CTIF (O43310, NP_055587). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago (100%); Marmoset, Mouse, Elephant, Panda, Bovine, Bat, Pig, Opossum, Turkey, Zebra finch, Lizard (92%); Xenopus (85%).
aa36-85
Human CTIF / KIAA0427
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About KIAA0427 / CTIF

O43310 NP_055587.1

CTIF Antibody, Gm672 Antibody, KIAA0427 Antibody

Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E.

Western blot

Western blot
CTIF / KIAA0427 antibody LS-C101872 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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