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Anti-KIAA0226 / RUBICON Antibody (aa241-271) LS-C156742

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular KIAA0226 / RUBICON Antibodies

Anti-KIAA0226 / RUBICON Antibody (aa311-360) LS-C116905
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Human, Monkey
Western blot
Unconjugated
Western blot Image
Anti-KIAA0226 / RUBICON Antibody (aa241-271) LS-C156742
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-KIAA0226 / RUBICON Antibody (aa249-278, AP) LS-C252385
Rabbit Polyclonal (IgG) to Human KIAA0226 / RUBICON
Human
IHC, Western blot, Flow Cytometry, ELISA
AP Conjugated
Anti-KIAA0226 / RUBICON Antibody (N-Terminus) LS-C336205
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-KIAA0226 / RUBICON Antibody LS-C410998
Rabbit Polyclonal (IgG) to Human KIAA0226 / RUBICON
Human
IHC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human KIAA0226 / RUBICON
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E5779 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156742. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa241-271
This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About KIAA0226 / RUBICON

Q92622 D86979 BAA13215.3

KIAA0226 Antibody, Baron Antibody, RUBICON Antibody, Rundataxin Antibody

KIAA0226 / RUBICON is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lymph with BARON Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with BARON antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BARON primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot

Western blot
Western blot of BARON antibody in MCF-7 cell line lysates (35 ug/lane). BARON (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric of MDA-231 cells using BARON Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/24/2017

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