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Catalog Number	Size	Price
LS-C156742-400	400 µl	$393

KIAA0226 / RUBICON Antibody - Formalin-fixed and paraffin-embedded human lymph with BARON Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

KIAA0226 / RUBICON Antibody - Fluorescent image of U251 cells stained with BARON antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BARON primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

KIAA0226 / RUBICON Antibody - Western blot of BARON antibody in MCF-7 cell line lysates (35 ug/lane). BARON (arrow) was detected using the purified antibody.

KIAA0226 / RUBICON Antibody - Flow cytometric of MDA-231 cells using BARON Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Polyclonal Rabbit anti‑Human KIAA0226 / RUBICON Antibody (aa241‑271, IHC, IF, WB) LS‑C156742

Name: Anti-KIAA0226 / RUBICON Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C156742-400
Price: 393.0000 USD
Availability: InStock

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Antibody:

KIAA0226 / RUBICON Rabbit anti-Human Polyclonal (aa241-271) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C156742-400

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

KIAA0226 / RUBICON Rabbit anti-Human Polyclonal (aa241-271) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

RUBICON antibody LS-C156742 is an unconjugated rabbit polyclonal antibody to human RUBICON (KIAA0226) (aa241-271). Validated for Flow, IF, IHC and WB.

Target

Human KIAA0226 / RUBICON

Synonyms

KIAA0226 | Baron | RUBICON | Rundataxin

See All KIAA0226 / RUBICON Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa241-271

Specificity

This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:100)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About KIAA0226 / RUBICON

Uniprot: Q92622 NCBI: D86979 BAA13215.3