MLEC antibody LS-A9847 is an unconjugated rabbit polyclonal antibody to MLEC (KIAA0152) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Human KIAA0152 / MLEC
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (5 µg/ml)
- (applications tested for the base form of this product only)
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KIAA0152 / MLEC antibody was raised against synthetic 15 amino acid peptide from N-terminus of human MLEC. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig, Pufferfish (100%); Chicken, Xenopus (87%); Stickleback, Tick (80%).
- Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A9847. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human MLEC. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A9847 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9847 was determined to be 5 ug/ml.
PBS, 0.1% Sodium Azide
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
For research use only.