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KF1 / RNF103 Antibody (clone 3E7) LS‑C198501
RNF103 antibody LS-C198501 is an unconjugated mouse monoclonal antibody to human RNF103 (KF1). Validated for ELISA, RNAi and WB.
Catalog
Size
Price
LS-C198501-100
100 µg
Unavailable

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Product Description

RNF103 antibody LS-C198501 is an unconjugated mouse monoclonal antibody to human RNF103 (KF1). Validated for ELISA, RNAi and WB.
About KF1 / RNF103
KF1 / RNF103 contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants. O00237 NM_005667 NP_005658.1

RNF103 Antibody, HKF-1 Antibody, ZFP-103 Antibody, ZFP103 Antibody, KF-1 Antibody, KF1 Antibody, Ring finger protein 103 Antibody


Specifications

Target
Human KF1 / RNF103
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3E7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • RNA interference
Immunogen
KF1 / RNF103 antibody was raised against rNF103 (NP_005658, aa501-599 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human KF1 / RNF103
Usage
RNAi Knockdown: Antibody validated. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.


Requested From: 
Date Requested: 6/22/2018

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