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| Catalog Number | Size | Price |
|---|---|---|
| LS-C71274-100 | 100 µl | $826 |
Monoclonal Mouse anti‑Human Keratan Sulfate Antibody (clone EFG‑11, WB) LS‑C71274
Monoclonal Mouse anti‑Human Keratan Sulfate Antibody (clone EFG‑11, WB) LS‑C71274
Note: This antibody replaces LS-C56340
Antibody:
Keratan Sulfate Mouse anti-Human Monoclonal (EFG-11) Antibody
Application:
WB, ELISA, Func, RIA
Reactivity:
Human, Mouse, Sheep
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Keratan Sulfate Mouse anti-Human Monoclonal (EFG-11) Antibody
Application:
WB, ELISA, Func, RIA
Reactivity:
Human, Mouse, Sheep
Format:
Unconjugated, Unmodified
Specifications
Description
Keratan Sulfate antibody LS-C71274 is an unconjugated mouse monoclonal antibody to Keratan Sulfate from human. It is reactive with human, mouse and sheep. Validated for ELISA, Func, RIA and WB.
Host
Mouse
Reactivity
Human, Mouse, Sheep
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
EFG-11
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Human articular cartilage proteoglycan digested with chondroitinase ABC.
Specificity
Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and is partially sensitive to keratanase or other endo-b-galactosidase treatment. Species cross-reactivity: Human, bovine, sheep, porcine, canine, mouse (adult). No cross-reactivity with chicken limb bud proteoglycans, dermatan sulfate proteoglycans of cartilage and proteoglycans of rat chondrosarcomas.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:2500 - 1:10000)
- Functional Assay
- Radioimmunoassay (1:15000 - 1:250000)
Usage
Suitable for use in Western Blot, ELISA and RIA. May be used in a competitive inhibition assay to measure keratan sulfate levels. Western Blot: 1:500-1:2000. ELISA: 1:2500-1:10000. RIA:1:15000-1:250000.
Presentation
No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
