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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KDM6B / JMJD3 Antibody (N‑Terminus) LS‑C144473
JMJD3 antibody LS-C144473 is an unconjugated rabbit polyclonal antibody to JMJD3 (KDM6B) from human, mouse and rat. Validated for ICC and WB.
Catalog
Size
Price
LS-C144473-100
100 µg (1 mg/ml)
Unavailable

Popular KDM6B / JMJD3 Products

Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of anti-JMJD3 C-term antibody in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.
Species: Mouse, Human
Applications: Western blot
KDM6B / JMJD3 antibody LS-C101819 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot
Antibody
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA

Product Description

JMJD3 antibody LS-C144473 is an unconjugated rabbit polyclonal antibody to JMJD3 (KDM6B) from human, mouse and rat. Validated for ICC and WB.
About KDM6B / JMJD3
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. O15054 AB002344 BAA21572.2

KDM6B Antibody, JMJD3 Antibody, Jumonji domain containing 3 Antibody, Lysine-specific demethylase 6B Antibody, KIAA0346 Antibody, Lysine demethylase 6B Antibody


Specifications

Target
Human KDM6B / JMJD3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (2.5 µg/ml)
  • Western blot (1 - 2 µg/ml)
Blocking Peptide
LS-E31221 - Liquid - 50 µg 0.2 mg/ml - $145.00
Immunizing peptide used to generate LS-C144473. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
This antibody is specific for JMJD3 and will not recognize other JMJD proteins.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.

Western blot

Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.
Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.

Western blot

Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.

Western blot

Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.
Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.

Western blot

Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.

Western blot

Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.
Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.

Western blot

Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.

Immunocytochemistry

Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.
Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 ug/ml.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.
Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 ug/ml.

Western blot

Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.
Western blot analysis of JMJD3 in HeLa cell lysate with JMJD3 antibody at 0.25 µg/mL.

Western blot

Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.


Requested From: 
Date Requested: 6/20/2018

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