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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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KCNN4 / KCa3.1 Antibody (C‑Terminus) LS‑C80110
KCa3.1 antibody LS-C80110 is an unconjugated rabbit polyclonal antibody to human KCa3.1 (KCNN4). Validated for WB.
Catalog
Size
Price
LS-C80110-50
50 µg (1 mg/ml)
Unavailable

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 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: Western blot

Product Description

KCa3.1 antibody LS-C80110 is an unconjugated rabbit polyclonal antibody to human KCa3.1 (KCNN4). Validated for WB.
About KCNN4 / KCa3.1
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. O15554 NM_002250 NP_002241.1

KCNN4 Antibody, HIKCa1 Antibody, HKCa4 Antibody, HIK1 Antibody, IK1 Antibody, KCA4 Antibody, HSK4 Antibody, IKCA1 Antibody, KCa3.1 Antibody, SK4 Antibody, SKCa 4 Antibody, Putative Gardos channel Antibody, SKCa4 Antibody


Specifications

Target
Human KCNN4 / KCa3.1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
KCNN4 / KCa3.1 antibody was raised against synthetic peptide from C-Terminus of human KCNN4 (O15554, NP_002241). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Horse (91%); Elephant, Panda (83%).
Epitope
C-Terminus
Specificity
Human KCNN4
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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