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KCNJ4 / Kir2.3 Antibody (C‑Terminus) LS‑C110000
Kir2.3 antibody LS-C110000 is an unconjugated rabbit polyclonal antibody to Kir2.3 (KCNJ4) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C110000-50
50 µg
$365

Popular KCNJ4 / Kir2.3 Products

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
KCNJ4 Antibody western blot of CEM cell line lysates (35 ug/lane). The KCNJ4 antibody detected the KCNJ4 protein (arrow).
Species: Human
Applications: Western blot
Anti-KCNJ4 / Kir2.3 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6727 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

Kir2.3 antibody LS-C110000 is an unconjugated rabbit polyclonal antibody to Kir2.3 (KCNJ4) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human KCNJ4 / Kir2.3
Synonyms
KCNJ4, IRK3, Kir2.3, HIR, HIRK2, HRK1, IRK-3, Hippocampal inward rectifier
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Guinea pig, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
KCNJ4 / Kir2.3 antibody was raised against synthetic peptide from C-Terminus of human KCNJ4 (P48050, NP_004972). Percent identity by BLAST analysis: Human, Gorilla, Galago, Mouse, Rat, Hamster, Panda, Dog, Rabbit, Pig, Guinea pig (100%); Elephant, Bat (92%).
Blocking Peptide
LS-E12508 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110000. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human KCNJ4 / Kir2.3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About KCNJ4 / Kir2.3
Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. P48050 NM_004981 NP_004972.1


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Images

Western blot

KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/22/2018

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