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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KCNJ4 / Kir2.3 Antibody (aa390‑445, clone S25‑35, Atto 700) LS‑C230727
Kir2.3 antibody LS-C230727 is an Atto 700-conjugated mouse monoclonal antibody to Kir2.3 (KCNJ4) from human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C230727-100
100 µg
$395

Popular KCNJ4 / Kir2.3 Products

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
KCNJ4 / Kir2.3 antibody LS-C110000 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Dog, Guinea pig, Hamster, Pig, Rabbit
Applications: Western blot
KCNJ4 Antibody western blot of CEM cell line lysates (35 ug/lane). The KCNJ4 antibody detected the KCNJ4 protein (arrow).
Species: Human
Applications: Western blot
Anti-KCNJ4 / Kir2.3 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6727 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

KCNJ4 / Kir2.3 Antibody (aa390-445, clone S25-35, Atto 700) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-C230727

Specifications

Target
Human KCNJ4 / Kir2.3
Synonyms
KCNJ4, IRK3, Kir2.3, HIR, HIRK2, HRK1, IRK-3, Hippocampal inward rectifier
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [S25-35]
Conjugations
Atto 700. Also available Unconjugated or conjugated with Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, AP, APC, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA, Atto 390, Atto 488.
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KCNJ4 / Kir2.3 antibody was raised against fusion protein amino acids 390-445 of human Kir2.3, accession number NP_032453.
Epitope
aa390-445
Specificity
Detects ~45kD. No cross-reactivity against Kir2.1 or Kir2.2.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About KCNJ4 / Kir2.3
Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. P48050 NM_004981 NP_004972.1


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Images

Immunohistochemistry

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Kir2.3 (S25-35), Human hippocampus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2.3 (S25-35), Human hippocampus. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Kir2 3 (S25-35), Human cell line mix.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2 3 (S25-35), Human cell line mix. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Kir2.3 (S25-35), Human hippocampus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2.3 (S25-35), Human hippocampus. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Kir2 3 (S25-35), Human cell line mix.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2 3 (S25-35), Human cell line mix. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Kir2.3 (S25-35), Human hippocampus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2.3 (S25-35), Human hippocampus. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Kir2 3 (S25-35), Human cell line mix.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2 3 (S25-35), Human cell line mix. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KCNJ4 / Kir2.3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7460 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Kir2.3 (S25-35), Human hippocampus.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2.3 (S25-35), Human hippocampus. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Kir2 3 (S25-35), Human cell line mix.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Kir2 3 (S25-35), Human cell line mix. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/20/2018

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