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Anti-KCNJ15 / KIR4.2 Antibody (clone 1B2) LS-C197575

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KCNJ15 / KIR4.2 Antibodies

Anti-KCNJ15 / KIR4.2 Antibody (aa338-367, APC) LS-C260929
Rabbit Polyclonal (IgG) to Human KCNJ15 / KIR4.2
Human
Western blot, ELISA
APC Conjugated
Anti-KCNJ15 / KIR4.2 Antibody (aa338-367, Azide-free, HRP) LS-C260933
Rabbit Polyclonal (IgG) to Human KCNJ15 / KIR4.2
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1B2] (IgG2a,k) to Human KCNJ15 / KIR4.2
Human
Western blot, ELISA, RNA interference
Unconjugated

Details

Human KCNJ15 / KIR4.2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1B2]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

KCNJ15 / KIR4.2 antibody was raised against kCNJ15 (NP_002234, aa290-355 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human KCNJ15 / KIR4.2
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About KCNJ15 / KIR4.2

Q99712 NM_002243 NP_002234.2

KCNJ15 Antibody, KIR1.3 Antibody, KIR4.2 Antibody, IRKK Antibody

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Western blot

Western blot
Western Blot analysis of KCNJ15 expression in transfected 293T cell line by KCNJ15 monoclonal antibody (M01), clone 1B2.Lane 1: KCNJ15 transfected lysate(42.6 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged KCNJ15 is 3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of KCNJ15 over-expressed 293 cell line, cotransfected with KCNJ15 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNJ15 monoclonal antibody (M01), clone 1B2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
 

Requested From: United States
Date Requested: 12/5/2016

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