Human KAT2B / PCAF

Rabbit

Human, Monkey, Mouse, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-Terminus) conjugated to KLH, corresponding to the C-Terminus amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%); Rat, Opossum, Platypus, Xenopus (94%); Chicken, Lizard, Zebrafish (88%); Stickleback, Pufferfish (81%).

aa817-832

Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.

• Western blot (1:500)
• Immunoprecipitation

Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500; Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 ug immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.

PBS, pH 7.4, 1% BSA, 15 mM sodium azide, 40% glycerol.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q92831 NCBI: NM_003884 NP_003875.3