Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Rat, Pig (at least 90% immunogen sequence identity)
Specificity and Use
KAT2A / GCN5 antibody was raised against synthetic peptide from human KAT2A / GCN5. Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%); Rat, Elephant, Pig (94%); Dog (89%); Mouse (83%).
Antibody has only been tested with human samples at this time.
Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following endurance exercise. Philp A, Chen A, Lan D, Meyer GA, Murphy AN, Knapp AE, Olfert IM, McCurdy CE, Marcotte GR, Hogan MC, Baar K, Schenk S. The Journal of biological chemistry. 2011 286:30561-70.
Reelin is a target of polyglutamine expanded ataxin-7 in human spinocerebellar ataxia type 7 (SCA7) astrocytes. McCullough SD, Xu X, Dent SY, Bekiranov S, Roeder RG, Grant PA. Proceedings of the National Academy of Sciences of the United States of America. 2012 109:21319-24.
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Hela cells stained with purified rabbit polyclonal antibody against GCN5 (poly6072), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-GCN5 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Requested From: United States
Date Requested: 10/21/2016