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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JUP/CTNNG/Junction Plakoglobin Antibody (clone PG‑11E4) LS‑C354880
Junction Plakoglobin antibody LS-C354880 is an unconjugated mouse monoclonal antibody to Junction Plakoglobin (JUP / CTNNG) from human and mouse. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C354880-100
100 µg
$395

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Product Description

Junction Plakoglobin antibody LS-C354880 is an unconjugated mouse monoclonal antibody to Junction Plakoglobin (JUP / CTNNG) from human and mouse. Validated for IF, IHC, IP and WB.
About JUP/CTNNG/Junction Plakoglobin
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. P14923 NM_002230 NP_002221.1

JUP Antibody, Desmoplakin-3 Antibody, CTNNG Antibody, DPIII Antibody, Gamma catenin Antibody, Junction plakoglobin Antibody, PKGB Antibody, ARVD12 Antibody, Catenin gamma Antibody, Desmoplakin III Antibody, DP3 Antibody, PDGB Antibody


Specifications

Target
Human JUP/CTNNG/Junction Plakoglobin
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [PG-11E4]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunofluorescence

Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)
WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)

Immunofluorescence

Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)
WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)

Immunofluorescence

Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)
WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)

Immunofluorescence

Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)
WB using Gamma-Catenin / Plakoglobin Antibody (PG-11E4)

Requested From: United States
Date Requested: 8/20/2018

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