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Anti-JUN / c-Jun Antibody (phospho-Thr91) LS-C14495

Ordering

Wt. Vol. Conc. Price
- 100 µl - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Sheep Polyclonal (IgG) to Human JUN / c-Jun
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated

Details

Human JUN / c-Jun
Sheep
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

Western blot (1:500 - 1:1000)

Specificity and Use

JUN / c-Jun antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 85-101 (NGHITT[pT]PTPTQFLCPK) of human c-jun. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Salmon, Zebrafish (100%); Trout, Stickleback, Pufferfish (90%); Trichina worm (80%).
pThr91
Recognizes phospho-c-Jun, Mr 41kD. An unknown protein was also detected, Mr 52kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with rat and bovine based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1:500-1:1000 detects phospho-c-Jun in RIPA lysates from 3T3 cells that had been treated with anisomycin. Untreated and anisomycin treated 3T3 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- c-Jun (1:500 dilution). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About JUN / c-Jun

P05412 NM_002228 NP_002219.1

JUN Antibody, Activator protein 1 Antibody, AP-1 Antibody, C-Jun Antibody, Jun proto-oncogene Antibody, p39 Antibody, Jun oncogene Antibody, Proto-oncogene c-Jun Antibody, AP1 Antibody, Enhancer-binding protein AP1 Antibody, G0s7 Antibody, Transcription factor AP-1 Antibody, v-jun Antibody

c-Jun is a component of the transcription factor activator protein 1 (AP-1) that binds to and activates transcription at TRE/AP-1 elements. Phosphorylation of c-Jun at Ser 63/73, resulting in activation and c-Jun dependent transcription, occurs in response to extra or intracellular signals, including UV irradiation, growth factors and transforming oncoproteins.

Requested From: United States
Date Requested: 9/26/2016

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