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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JUN / c‑Jun Antibody (aa221‑270) LS‑C117861
C-Jun antibody LS-C117861 is an unconjugated rabbit polyclonal antibody to c-Jun (JUN) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C117861-50
50 µl
Unavailable
LS-C117861-100
100 µl
Unavailable

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Western blot of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Applications: IHC, Western blot

Product Description

C-Jun antibody LS-C117861 is an unconjugated rabbit polyclonal antibody to c-Jun (JUN) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About JUN / c-Jun
c-Jun is a component of the transcription factor activator protein 1 (AP-1) that binds to and activates transcription at TRE/AP-1 elements. Phosphorylation of c-Jun at Ser 63/73, resulting in activation and c-Jun dependent transcription, occurs in response to extra or intracellular signals, including UV irradiation, growth factors and transforming oncoproteins. P05412 NM_002228 NP_002219.1

JUN Antibody, Activator protein 1 Antibody, AP-1 Antibody, C-Jun Antibody, Jun proto-oncogene Antibody, p39 Antibody, Jun oncogene Antibody, Proto-oncogene c-Jun Antibody, AP1 Antibody, Enhancer-binding protein AP1 Antibody, G0s7 Antibody, Transcription factor AP-1 Antibody, v-jun Antibody


Specifications

Target
Human JUN / c-Jun
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
JUN / c-Jun antibody was raised against synthetic peptide from human c-Jun (aa221-270).
Epitope
aa221-270
Specificity
c-Jun Antibody detects endogenous levels of total c-Jun protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human brain tissue, using c-Jun (Ab-249) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from Jurkat cells, treated with UV 15', using c-Jun Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/17/2018

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