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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JCHAIN / Ig J Chain Antibody (clone 2B1) LS‑C173679
Ig J Chain antibody LS-C173679 is an unconjugated mouse monoclonal antibody to human Ig J Chain (JCHAIN). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C173679-100
100 µl
Unavailable

Popular JCHAIN / Ig J Chain Products

Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

Ig J Chain antibody LS-C173679 is an unconjugated mouse monoclonal antibody to human Ig J Chain (JCHAIN). Validated for Flow, IHC and WB.
About JCHAIN / Ig J Chain
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component. P01591 M12759 AAA58902.1

JCHAIN Antibody, IGCJ Antibody, Immunoglobulin J chain Antibody, IGJ Antibody, IgJ chain Antibody, JCH Antibody


Specifications

Target
Human JCHAIN / Ig J Chain
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2B1]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human IGJ(NP_653247) produced in HEK293T cell.
Specificity
Human IGJ
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-IGJ mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-IGJ antibody (Red), compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/23/2018

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