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Anti-JAG1 / Jagged 1 Antibody (aa110-125) LS-C18929

Ordering

Wt. Vol. Conc. Price
500 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular JAG1 / Jagged 1 Antibodies

Anti-JAG1 / Jagged 1 Antibody (aa110-125) LS-C71129
Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-JAG1 / Jagged 1 Antibody (aa110-125) IHC-plus™ LS-B2442
Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human JAG1 / Jagged 1
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human JAG1 / Jagged 1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)

  • IHC - Paraffin

Specificity and Use

JAG1 / Jagged 1 antibody was raised against synthetic peptide from human JAG1 / Jagged 1.
aa110-125
Amino acids 110-125 of human Jagged-1protein.
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kD band in human brain and kidney lysates.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

About JAG1 / Jagged 1

P78504 NM_000214 NP_000205.1

JAG1 Antibody, AGS Antibody, AHD Antibody, Alagille syndrome Antibody, CD339 antigen Antibody, HJ1 Antibody, Jagged1 Antibody, JAGL1 Antibody, Jagged Antibody, Jagged 1 Antibody, Protein jagged-1 Antibody, AWS Antibody, CD339 Antibody, Soluble protein jagged Antibody

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry - Jagged-1 Antibody.

Immunofluorescence

Immunofluorescence
Immunofluorescence - Jagged-1 Antibody. Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.

Western blot

Western blot
Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.
 

Requested From: United States
Date Requested: 12/4/2016

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