Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone SY5] (IgG1) to Human IVL / Involucrin
Human, Monkey, Dog, Pig
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Human IVL / Involucrin
Human, Monkey, Dog, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [SY5]
Protein A purified
IHC - Paraffin (10 µg/ml)
IHC - Frozen
Specificity and Use
IVL / Involucrin antibody was raised against native, from keratinocytes.
Recognizes human involucrin, a 585 amino acid soluble protein encoded by the IVL gene and expressed in epidermal keratinocytes as they tend towards terminal differentiation. Involucrin is produced in the stratum spinosum initially in the cell cytosol from where it moves to the cell membrane, associates with transglutaminase and becomes cross-linked to membrane proteins, offering structural support to the cell and forming a stable envelope below the plasma membrane. Recognizes an epitope between residues 421-568 of human involucrin. Involucrin expression is seen in the normal granular cells of the epidermis associated with the plasma membrane during the early phase of cornified envelope maturation and expression is increased in psoriatic epidermis with abnormal cornified envelope maturation. Involucrin staining is also maintained, associated with the cornified envelope in epidermolytic hyperkeratosis, a condition associated with mutations to mutations in genes coding for cytokeratins 1 and 10.
PBS, 0.09% sodium azide. Sourced in TCS.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.