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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ITGB5 / Integrin Beta 5 Antibody (clone 2C4) LS‑C197557
Integrin Beta 5 antibody LS-C197557 is an unconjugated mouse monoclonal antibody to human Integrin Beta 5 (ITGB5). Validated for ELISA, RNAi and WB.
Catalog
Size
Price
LS-C197557-100
100 µg (0.5 mg/ml)
Unavailable

Popular ITGB5 / Integrin Beta 5 Products

Antibody
Species: Human, Mouse, Dog
Applications: ICC, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-ITGB5 / Integrin Beta 5 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse
Applications: ICC, Western blot

Product Description

Integrin Beta 5 antibody LS-C197557 is an unconjugated mouse monoclonal antibody to human Integrin Beta 5 (ITGB5). Validated for ELISA, RNAi and WB.
About ITGB5 / Integrin Beta 5
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. P18084 NM_002213 NP_002204.2

ITGB5 Antibody, Integrin Beta 5 Antibody, Integrin beta-5 Antibody, Integrin beta-5 subunit Antibody, Integrin, beta 5 Antibody


Specifications

Target
Human ITGB5 / Integrin Beta 5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2C4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • RNA interference
Immunogen
ITGB5 (NP_002204, aa421-516 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human ITGB5 / Integrin Beta 5
Usage
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.

Western blot

ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.
ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.

Western blot

ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.
ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.

Western blot

ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.
ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.

Western blot

ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.
ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ITGB5 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.


Requested From: 
Date Requested: 6/21/2018

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