Human ITGB4 / Integrin Beta 4

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Human squamous cell carcinoma cells (SCC9).

Human b4 integrin (CD104).

• IHC
• IHC - Frozen
• Immunoprecipitation
• Flow Cytometry
• Functional Assay

Suitable for use in Immunohistochemistry, Flow Cytometry, Inhibition of Cell Attachment and Immunoprecipitation. Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.

PBS, pH 7.6, 0.1% Sodium Azide

Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16144 NCBI: NM_000213 NP_000204.3