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Anti-ITGB4 / Integrin Beta 4 Antibody (clone 439-9B) IHC-plus™ LS-B3778

Note: This antibody replaces LS-C106888

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ITGB4 / Integrin Beta 4 Antibodies

Anti-ITGB4 / Integrin Beta 4 Antibody (clone 450-9D, Azide-free) LS-C14106
Mouse Monoclonal [clone 450-9D] (IgG1) to Human ITGB4 / Integrin Beta 4
Human
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Anti-ITGB4 / Integrin Beta 4 Antibody (clone 439-9B) IHC-plus™ LS-B3778
Rat Monoclonal [clone 439-9B] (IgG2b) to Human ITGB4 / Integrin Beta 4
Human
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ITGB4 / Integrin Beta 4 Antibody (aa1619-1822, clone 10B10D5) IHC-plus™ LS-B9845
Mouse Monoclonal [clone 10B10D5] (IgG1) to Human ITGB4 / Integrin Beta 4
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 439-9B] (IgG2b) to Human ITGB4 / Integrin Beta 4
Human
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Unconjugated

Details

Human ITGB4 / Integrin Beta 4
Rat
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [439-9B]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen
  • Western blot
  • Flow Cytometry

Specificity and Use

ITGB4 / Integrin Beta 4 antibody was raised against human ITGB4
Immunohistochemistry: LS-B3778 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3778 was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging

aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About ITGB4 / Integrin Beta 4

P16144 NM_000213 NP_000204.3

ITGB4 Antibody, CD104 Antibody, Integrin beta-4 Antibody, Integrin Beta 4 Antibody, gp150 Antibody, CD104 antigen Antibody, Integrin beta-4 subunit Antibody, Integrin, beta 4 Antibody

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins.

Immunohistochemistry

Immunohistochemistry
Anti-Integrin Beta 4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3778 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Integrin Beta 4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3778 concentration 20 ug/ml.

Flow Cytometry

Flow Cytometry
Staining of the A549 cell line with 0.5 ug of Purified Rat IgG2b Isotype Control (open histogram) or 0.5 ug of Purified anti-human CD104 (439-9B) (colored histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.
 

Requested From: United States
Date Requested: 12/2/2016

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