Human ITGB1 / Integrin Beta 1 / CD29

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Human IL-1b activated endothelial cells.

Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.

• IHC
• IHC - Paraffin
• IHC - Frozen
• ICC (2 µg/ml)
• Immunoprecipitation
• Flow Cytometry
• ELISA

Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 ug/ml (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 ug/400 ul lysate (0.5% triton PBS plus Ca++). FACS: 2-5 ug/1E6 cells, in the presence of Ca++. Stimulation of cells: 2-10 ug/ml per 1E6 cells. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

PBS, pH 7.1. No preservatives.

Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05556 NCBI: NM_002211 NP_002202.2