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Anti-ITGAM / CD11b Antibody (FITC) LS-C45331
Note: This antibody replaces LS-C58657Most Popular ITGAM / CD11b Antibodies
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Details
| Mouse ITGAM / CD11b | |
| Rat | |
| Mouse, Human, Rabbit (tested or 100% immunogen sequence identity) | |
| IgG2b Monoclonal | |
| Fluorescein | |
| Protein A purified | |
| Unmodified |
Applications
| Flow Cytometry (1:1 - 1:10) |
Specificity and Use
| ITGAM / CD11b antibody was raised against t cell enriched splenocytes from B10 mice. | |
| Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This product is routinely tested in flow cytometry on mouse peritoneal macrophages. | |
| Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Packaging
| PBS, pH 7.4, 0.09% sodium azide, 1% BSA. | |
| For research use only. |
About ITGAM / CD11b
P11215
NM_000632
NP_000623.2
ITGAM Antibody, Antigen CD11b (p170) Antibody, CD11B Antibody, CD11b antigen Antibody, CR3A Antibody, Integrin alpha-M Antibody, MAC-1 Antibody, MO1A Antibody, Neutrophil adherence receptor Antibody, MAC1A Antibody, SLEB6 Antibody, CR-3 alpha chain Antibody
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
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Requested From:
Date Requested: 4/24/2018
Date Requested: 4/24/2018
