(tested or 100% immunogen sequence identity)
- IHC - Frozen (1:100 - 1:200)
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ITGAM / CD11b antibody was raised against peritoneal macrophages
CD11b/c. OX-42 recognizes three major polypeptides of 160kDa, 103kDa and 95kDa and a minor 133kDa protein under non-reducing conditions. Under reducing conditions two major peptides of 163kDa and 100kDa and a minor 135kDa peptide are detected. Antigen distribution: Isolated cells: OX-42 shows a similar reaction pattern to OX-41 on various isolated cells but stains bronchial lavage cells to a lesser degree. OX-42 inhibits complement mediated rosettes. Tissue sections: OX-42 is a most suitable marker for the identification of microglial cells and Kupffer cells. It also stains dendritic cells strongly. Only a subpopulation of alveolar macrophages is recognised. Specificity: Rat: granulocytes, macrophages, microglial cells. Other species: not tested
PBS pH 7.2, 0.09% sodium azide
For research use only.
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