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Anti-ITGAE / CD103 Antibody (clone AX.14, PE) LS-C140408

Ordering

Wt. Vol. Conc. Price
- 100 tst - $305
Inquire for larger quantities

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Most Popular ITGAE / CD103 Antibodies

Anti-ITGAE / CD103 Antibody (aa1153-1168) LS-C312777
Rabbit Polyclonal (IgG) to Human ITGAE / CD103
Human, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AX.14] (IgG1) to Human ITGAE / CD103
Human
Flow Cytometry
PE Conjugated

Details

Human ITGAE / CD103
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AX.14]
Phycoerythrin
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

Human ITGAE / CD103
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.

About ITGAE / CD103

P38570 NM_002208 NP_002199.3

ITGAE Antibody, CD103 Antibody, CD103 antigen Antibody, Integrin alpha-E Antibody, HML-1 antigen Antibody, Integrin alpha-IEL Antibody, Alpha polypeptide) Antibody, Mucosal lymphocyte 1 antigen Antibody, HUMINAE Antibody

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen.

Requested From: United States
Date Requested: 12/6/2016

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