Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1. Stepp MA, Liu Y, Pal-Ghosh S, Jurjus RA, Tadvalkar G, Sekaran A, Losicco K, Jiang L, Larsen M, Li L, Yuspa SH. Journal of cell science. 2007 120:2851-63.
Rare steroid receptor-negative basal-like tumorigenic cells in luminal subtype human breast cancer xenografts. Horwitz KB, Dye WW, Harrell JC, Kabos P, Sartorius CA. Proceedings of the National Academy of Sciences of the United States of America. 2008 105:5774-9. (IHC)
Differential requirement of mTOR in postmitotic tissues and tumorigenesis. Nardella C, Carracedo A, Alimonti A, Hobbs RM, Clohessy JG, Chen Z, Egia A, Fornari A, Fiorentino M, Loda M, Kozma SC, Thomas G, Cordon-Cardo C, Pandolfi PP. Science signaling. 2009 2:ra2.
Reprogramming murine telomerase rapidly inhibits the growth of mouse cancer cells in vitro and in vivo. Xu T, Xu Y, Liao CP, Lau R, Goldkorn A. Molecular cancer therapeutics. 2010 9:438-49.
Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Jo M, Eastman BM, Webb DL, Stoletov K, Klemke R, Gonias SL. Cancer research. 2010 70:8948-58.
Microvesicles released from human renal cancer stem cells stimulate angiogenesis and formation of lung premetastatic niche. Grange C, Tapparo M, Collino F, Vitillo L, Damasco C, Deregibus MC, Tetta C, Bussolati B, Camussi G. Cancer research. 2011 71:5346-56.
Suppressed prostate epithelial development with impaired branching morphogenesis in mice lacking stromal fibromuscular androgen receptor. Lai KP, Yamashita S, Vitkus S, Shyr CR, Yeh S, Chang C. Molecular endocrinology (Baltimore, Md.). 2012 26:52-66.
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
MDA-MB-231 breast cancer cells were stained with anti-CD49f (clone GoH3) followed by DyLight 649 Goat anti-rat Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC