Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein G purified
Immunoprecipitation (25 - 50 µg/ml)
Flow Cytometry (1:25 - 1:50)
ELISA (10 µg/ml)
IHC - Paraffin
Specificity and Use
ITGA2 / CD49b antibody was raised against purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
31H4 has been identified as being capable of immunoprecipitating the integrin a2 subunit from human cell-lines surface labeled with 125I. It has been confirmed specific to a2 by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified a2b1 in dot blots.
ITGA2 encodes integrin alpha chain 2. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.