Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Hamster Monoclonal [clone HMa2] (IgG) to Mouse ITGA2 / CD49b
Immunoprecipitation, Flow Cytometry
Mouse ITGA2 / CD49b
Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [HMa2]
Specificity and Use
ITGA2 / CD49b antibody was raised against mouse ITGA2
The HMα2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
ITGA2 encodes integrin alpha chain 2. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Staining of BALB/c splenocytes with 0.25 ug of Purified Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Purified anti-mouse CD49b (HMa2) (colored histogram) followed by FITC Anti-Armenian Hamster IgG. Cells in the lymphocyte gate were used for analysis.
Requested From: United States
Date Requested: 1/21/2017