Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [16B4]
Protein G purified
IHC - Paraffin
IHC - Frozen (30 - 40 µg/ml)
Immunoprecipitation (25 - 50 µg/ml)
Flow Cytometry (30 µg/ml)
ELISA (10 µg/ml)
Specificity and Use
ITGA2 / CD49b antibody was raised against purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
Is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA pH9.0 is recommended for this purpose.
PBS, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
ITGA2 encodes integrin alpha chain 2. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.