Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey (tested or 100% immunogen sequence identity)
Protein G purified
Flow Cytometry (1:50 - 1:100)
Specificity and Use
Recognizes the integrin alpha 2 subunit, which is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. Inhibits cell attachment to collagen. Species cross-reactivity: cross-reacts with Cynomolgus monkey, Baboon and Rhesus monkey.
Flow Cytometry: 1:50-1:100. Use 10 ul of the suggested working dilution to label 1E6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
ITGA2 encodes integrin alpha chain 2. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.