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Anti-ITGA1/CD49a/Integrin Alpha 1 Antibody LS-C149978

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

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Most Popular ITGA1/CD49a/Integrin Alpha 1 Antibodies

Anti-ITGA1/CD49a/Integrin Alpha 1 Antibody LS-C36920
Mouse Monoclonal (IgG1) to Human ITGA1/CD49a/Integrin Alpha 1
Human
IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Functional Assay
Unconjugated
Anti-ITGA1/CD49a/Integrin Alpha 1 Antibody LS-C149978
Sheep Polyclonal (IgG) to Human ITGA1/CD49a/Integrin Alpha 1
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

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Sheep Polyclonal (IgG) to Human ITGA1/CD49a/Integrin Alpha 1
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human ITGA1/CD49a/Integrin Alpha 1
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

ITGA1/CD49a/Integrin Alpha 1 antibody was raised against chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha1/CD49a Phe29-Pro1 141 Accession # P56199.
Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant.
Western Blot, 1 µg/ml. Flow Cytometry, 2.5 µg/106 cells. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
500 µl Sterile PBS
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About ITGA1/CD49a/Integrin Alpha 1

P56199 NM_181501 NP_852478.1

ITGA1 Antibody, CD49a antigen Antibody, CD49a Antibody, Integrin Alpha 1 Antibody, Integrin, alpha 1 Antibody, Integrin alpha-1 Antibody, Laminin and collagen receptor Antibody, Very late activation protein 1 Antibody, VLA1 Antibody, VLA-1 Antibody

The integrin alpha-1 chain associates with the integrin beta-1 chain to form a heterodimeric cell surface unit that functions as a receptor for laminin and collagen in neural cells and hematopoietic cells. It is involved in cell adhesion and neurite outgrowth. It may also play a role in inflammation and fibrosis.

Immunohistochemistry

Immunohistochemistry
Integrin alpha 1/CD49a in Human Stomach Cancer Tissue. Integrin alpha 1/CD49a was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Sheep Anti-Human Integrin alpha 1/CD49a Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Integrin a1/CD49a in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin a1/CD49a Antigen Affinity-purgied Polyclonal Antobody (filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.

Flow Cytometry

Flow Cytometry
Detection of Integrin alpha 1/CD49a in He La Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin alpha 1/CD49a Antigen Affinity-purified Polyclonal Antibody or isotype control antibody, followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.
 

Requested From: United States
Date Requested: 12/3/2016

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