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Anti-IRS4 Antibody (aa633-647) LS-C186534

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IRS4 Antibodies

Anti-IRS4 Antibody (aa633-647) LS-C186534
Goat Polyclonal to Mouse IRS4
Mouse
Western blot, ELISA
Unconjugated
Western blot Image
Anti-IRS4 Antibody (aa850-951, APC) LS-C278560
Mouse Monoclonal (IgG1) to Human IRS4
Human
IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
APC Conjugated
Anti-IRS4 Antibody (aa850-951, Azide-free, HRP) LS-C278563
Mouse Monoclonal (IgG1) to Human IRS4
Human
IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Mouse IRS4
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse IRS4
Goat
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 0.6 µg/ml)
  • ELISA (1:128000)

Specificity and Use

IRS4 antibody was raised against synthetic peptide C-DRGTKERKEAKEVRD from an internal region of mouse IRS4 (NP_034702.2). Percent identity by BLAST analysis: Mouse (100%).
LS-E27999 - Lyophilized - 100 µg - $145.00
aa633-647
Mouse IRS4
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 150kD band observed in Mouse Heart lysates (calculated MW of 130kD according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6 ug/ml. An additional band of unknown identity was also consistently observed at ~39kD. This band was successfully blocked by incubation with the immunizing peptide.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About IRS4

O14654 NM_003604 NP_003595.1

IRS4 Antibody, Insulin receptor substrate 4 Antibody, Phosphoprotein of 160 kDa Antibody, IRS-4 Antibody, Pp160 Antibody, PY160 Antibody

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis.

Western blot

Western blot
IRS4 antibody (0.2 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/10/2016

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