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Anti-IRS4 Antibody (aa1240-1257) LS-C8288


Wt. Vol. Conc. Price
200 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IRS4
Western blot, Immunoprecipitation


Human IRS4
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • Western blot
  • Immunoprecipitation

Specificity and Use

IRS4 antibody was raised against kLH conjugated synthetic peptide (C-RMDFARRDNQFDSPKRGR) corresponding to amino acids 1240-1257 of human IRS-4. The immunizing sequence does not share any significant homology with IRS-1, IRS-2 or IRS-3. Percent identity by BLAST analysis: Human (100%); Gibbon (94%); Monkey (89%); Bovine (83%).
Recognizes IRS-4, Mr 133kD-160kD, due to multiple phosphorylation sites. Specificity for IRS-4 was demonstrated by using the immunizing peptide to inhibit antibody binding. Species cross-reactivity: Human and rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 0.5-2.0 ug/ml detected IRS-4 in RIPA lysates from human embryonic kidney (HEK) cells and rat L6 cells. The IRS-4 protein band appears to run at a slightly lower MWapp on minigels than is reported in literature. HEK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-rabbit IRS-4 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated IRS-4 from 500 ug of human embryonic kidney RIPA lysate.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IRS4

O14654 NM_003604 NP_003595.1

IRS4 Antibody, Insulin receptor substrate 4 Antibody, Phosphoprotein of 160 kDa Antibody, IRS-4 Antibody, Pp160 Antibody, PY160 Antibody

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis.

Requested From: 
Date Requested: 4/26/2017

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