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Anti-IRS2 / IRS-2 Antibody (aa1309-1338) LS-C96725

Catalog Size Price
LS-C96725-400 400 µl Unavailable

Related Products

22 IRS2 / IRS-2 Antibodies

Most Popular IRS2 / IRS-2 Antibodies

Anti-IRS2 / IRS-2 Antibody (phospho-Ser731) LS-C33929
Rabbit Polyclonal to Human IRS2 / IRS-2
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Western blot, Immunoprecipitation, ELISA
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Anti-IRS2 / IRS-2 Antibody (Ser731) LS-C33930
Rabbit Polyclonal to Human IRS2 / IRS-2
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Anti-IRS2 / IRS-2 Antibody LS-C62152
Mouse Monoclonal (IgG1,k) to Human IRS2 / IRS-2
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Western blot Image
Anti-IRS2 / IRS-2 Antibody (aa1100-1150) LS-C288228
Rabbit Polyclonal (IgG) to Human IRS2 / IRS-2
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Anti-IRS2 / IRS-2 Antibody (aa1288-1338) IHC-plus™ LS-B11049
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Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human IRS2 / IRS-2
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human IRS2 / IRS-2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

  • IHC - Paraffin

Specificity and Use

LS-E9260 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C96725. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa1309-1338
This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About IRS2 / IRS-2

Q9Y4H2 NM_003749 NP_003740.2

IRS2 Antibody, Insulin receptor substrate 2 Antibody, IRS-2 Antibody

IRS2 / IRS-2 is the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent image of HeLa cell stained with IRS2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). IRS2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
Western blot of anti-IRS2 Antibody in 293 cell line lysates (35 ug/lane). IRS2 (arrow) was detected using the purified antibody.

Requested From: 
Date Requested: 8/23/2017

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