(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
IRS1 antibody was raised against synthetic phosphopeptide corresponding to residues surrounding Ser612 of mouse IRS-1 (KLH coupled).
Detects endogenous IRS-1 only when phosphorylated at serine 612 of mouse IRS-1 or serine 616 of human IRS-1. Does not detect IRS-1 phosphorylated at other sites. Species cross-reactivity: Human. Expected to cross-react with rat based on sequence homology.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.