Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:2000)
IRS1 antibody was raised against synthetic peptide corresponding to aa301-315 (ESITATpSPASMVGGK) of phospho-IRS-1 (Ser307) (KLH coupled).
Recognizes phospho-IRS-1 (Ser307), Mr 170kD. Species cross-reactivity: Human, mouse and rat. Species sequence homology: mouse, human, rat and monkey:100%; chicken: 12/15.
Suitable for use in Western Blot. Western Blot: 1:1000-1:2000 detects phospho-IRS-1 (Ser307) in RIPA lysates from serum starved HEK 293 cells treated with 5 ug/ml anisomycin for 30 minutes, but not in untreated cells.
TBS, 0.05% sodium azide. Glycerol free.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.