IRS1 antibody LS-C8275 is an unconjugated rabbit polyclonal antibody to IRS1 from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:2000)
IRS1 antibody was raised against synthetic peptide corresponding to aa301-315 (ESITATpSPASMVGGK) of phospho-IRS-1 (Ser307) (KLH coupled).
Recognizes phospho-IRS-1 (Ser307), Mr 170kD. Species cross-reactivity: Human, mouse and rat. Species sequence homology: mouse, human, rat and monkey:100%; chicken: 12/15.
Suitable for use in Western Blot. Western Blot: 1:1000-1:2000 detects phospho-IRS-1 (Ser307) in RIPA lysates from serum starved HEK 293 cells treated with 5 ug/ml anisomycin for 30 minutes, but not in untreated cells.
TBS, 0.05% sodium azide. Glycerol free.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.