(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
IRS1 antibody was raised against synthetic phosphopeptide corresponding to residues surrounding Ser307 of mouse IRS-1 (KLH coupled). Equivalent to human Ser312.
Detects overexpressed or endogeneous mouse IRS-1 only when phosphorylated at Ser307 (Mr 175kD). Does not cross-react with other related phospho-proteins. Species cross-reactivity: Predicted to cross-react with human based on sequence homology.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50.
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.