Target
Rat IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the C-Terminus of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
Specificity
Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates IRS-1 from 500 ug of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.
Presentation
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/ml BSA, 0.05% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee