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| Catalog Number | Size | Price |
|---|---|---|
| LS-C8262-100 | 100 µg | $826 |
Polyclonal Rabbit anti‑Rat IRS1 Antibody (aa1222‑1235, WB) LS‑C8262
Polyclonal Rabbit anti‑Rat IRS1 Antibody (aa1222‑1235, WB) LS‑C8262
Antibody:
IRS1 Rabbit anti-Rat Polyclonal (aa1222-1235) Antibody
Application:
WB, IP
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IRS1 Rabbit anti-Rat Polyclonal (aa1222-1235) Antibody
Application:
WB, IP
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Specifications
Description
IRS1 antibody LS-C8262 is an unconjugated rabbit polyclonal antibody to rat IRS1 (aa1222-1235). Validated for IP and WB.
Target
Rat IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Rabbit
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the C-Terminus of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
Epitope
aa1222-1235
Specificity
Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates IRS-1 from 500 ug of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.
Presentation
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/ml BSA, 0.05% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
