(tested or 100% immunogen sequence identity)
Human, Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
- Western blot (0.5 - 2 µg/ml)
IRS1 antibody was raised against synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the carboxy terminal of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates IRS-1 from 500 ug of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/ml BSA, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.