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| Catalog Number | Size | Price |
|---|---|---|
| LS-C8283-50 | 50 µg | $528 |
Polyclonal Rabbit anti‑Rat IRS1 Antibody (Biotin, aa1222‑1235, WB) LS‑C8283
Polyclonal Rabbit anti‑Rat IRS1 Antibody (Biotin, aa1222‑1235, WB) LS‑C8283
Antibody:
IRS1 Rabbit anti-Rat Polyclonal (aa1222-1235) (Biotin) Antibody
Application:
WB, IP
Reactivity:
Rat
Format:
Biotin, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IRS1 Rabbit anti-Rat Polyclonal (aa1222-1235) (Biotin) Antibody
Application:
WB, IP
Reactivity:
Rat
Format:
Biotin, Unmodified
Specifications
Description
IRS1 antibody LS-C8283 is a biotin-conjugated rabbit polyclonal antibody to rat IRS1 (aa1222-1235). Validated for IP and WB.
Target
Rat IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Rabbit
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Biotin
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Carboxy terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
Epitope
aa1222-1235
Specificity
Recognizes rat IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRS-1 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates IRS-1 from 500 ug of 3T3/A31 RIPA lysate. Positive control: 3T3/A31 cell lysate. Load 20 ug of reduced lysate per lane for minigels. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
