Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
Specificity and Use
IRS1 antibody was raised against carboxy-terminal 14 amino acid peptide of rat liver IRS-1 (C-YASINFQKQPEDRQ). Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
Recognizes rat IRS-1.
Immunoprecipitation: 50 ul immunoprecipitates IRS-1 from 500 ug of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer a small amount of the non-covalently bound IgG light chain (28kD) and heavy chain (55kD) may be observed. Elution Buffer % IRS-1 Released SDS-Page sample buffer 100 0.5 M Glycine, pH 2, 0 0.1 M TEA, pH 11.5. Affinity Purification: The antibody-agarose may be useful for affinity purification of proteins that bind IRS-1.
50% gel slurry in PBS, 0.05% azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.